Not all codons are decoded at the same rate Not all codons are decoded at the same rate; some are translated quickly, while others may cause ribosomes to pause or move slower. These differences in decoding rates can be influenced by factors such as codon sequence, tRNA abundance and mRNA structure. Ribosome profiling (Ribo-seq) maps […]

https://eirnabio.com/wp-content/uploads/2025/08/Question-29.mp4 Introduction Ribosome profiling (Ribo-seq)’s capture of the precise location and density of ribosomes across the entire transcriptome provides a snapshot of actively translated regions. Recent research has leveraged Ribo-seq’s inherent triplet periodicity signal and mRNA positional information to predict the translation of open reading frames (ORFs) alternative to the annotated protein coding ORF (CDS)1,2. […]

https://eirnabio.com/wp-content/uploads/2025/08/Question-5.mp4 Introduction Insight into translational efficiency Polysome profiling allows monitoring of mRNA translation activity using sucrose gradient fractionation. The sequencing of the collected polysome fractions offers two key advantages over Ribo-seq: (1) it enables the quantification of the exact number of ribosomes per mRNA, and (2) the longer reads of polysome-seq allow for more accurate […]

Introduction In the dynamic landscape of molecular biology, differential gene expression (DE) analysis serves as a cornerstone for understanding the complex regulatory mechanisms underpinning biological processes and disease states. Two revolutionary techniques, ribosome profiling (Ribo-seq) and RNA sequencing (RNA-seq), have emerged as powerful tools for dissecting the nuances of gene expression at an unprecedented resolution. […]