https://eirnabio.com/wp-content/uploads/2025/08/Question-5.mp4 Introduction Insight into translational efficiency Polysome profiling allows monitoring of mRNA translation activity using sucrose gradient fractionation. The sequencing of the collected polysome fractions offers two key advantages over Ribo-seq: (1) it enables the quantification of the exact number of ribosomes per mRNA, and (2) the longer reads of polysome-seq allow for more accurate […]

Introduction In the dynamic landscape of molecular biology, differential gene expression (DE) analysis serves as a cornerstone for understanding the complex regulatory mechanisms underpinning biological processes and disease states. Two revolutionary techniques, ribosome profiling (Ribo-seq) and RNA sequencing (RNA-seq), have emerged as powerful tools for dissecting the nuances of gene expression at an unprecedented resolution. […]

Respiratory illnesses encompass a wide range of disorders affecting the lungs and other parts of the respiratory system. These illnesses can vary from acute conditions like the common cold to chronic diseases such as asthma, lung cancer and as seen in recent times, SARS-CoV-2. The impact of respiratory illnesses on global health is profound, with millions […]

Melanoma is a particularly aggressive cancer, well known for its ability to rapidly metastasize to other organs, making early diagnosis crucial. Alarmingly, it is also currently one of the fast growing cancers in the Western world (1), and so further research in this area is particularly important. Control of protein synthesis in cancer is crucial, […]

Viruses, lacking their own cellular organelles, necessarily hijack its host’s translational machinery, namely the ribosome, to produce the proteins required for replication and propagation. Additionally, many viruses utilise the recoding phenomenon of frameshifting to significantly increase the genomic information within their small genomes. As such, translatomics is a key area of study in the field […]