https://eirnabio.com/wp-content/uploads/2025/08/Question-5.mp4 Introduction Insight into translational efficiency Polysome profiling allows monitoring of mRNA translation activity using sucrose gradient fractionation. The sequencing of the collected polysome fractions offers two key advantages over Ribo-seq: (1) it enables the quantification of the exact number of ribosomes per mRNA, and (2) the longer reads of polysome-seq allow for more accurate […]
Introduction In the dynamic landscape of molecular biology, differential gene expression (DE) analysis serves as a cornerstone for understanding the complex regulatory mechanisms underpinning biological processes and disease states. Two revolutionary techniques, ribosome profiling (Ribo-seq) and RNA sequencing (RNA-seq), have emerged as powerful tools for dissecting the nuances of gene expression at an unprecedented resolution. […]
https://eirnabio.com//srv/htdocs/wp-content/uploads/2023/06/Question-40-1.mp4 In the realm of drug discovery, understanding the mechanism of action of therapeutic compounds is crucial, and ribosome profiling (Ribo-seq) has emerged as a powerful tool for this purpose. Despite its strengths, the application of Ribo-seq in a high-throughput manner poses practical limitations. The resource-intensive nature of the technology makes it challenging to triage […]
Recent Publications Harnessing the Power of Translatomics Every week we provide a digest of a small number of recent interesting papers in the field of translatomics. In this week’s Sunday papers, Ando et al. show that tissue-specific tRNA modifications influence codon decoding and translation efficiency. Scazzari et al. demonstrate that stalled disomes are marked by Hel2-dependent ubiquitin chains. […]
Recent Publications Harnessing the Power of Translatomics Every week we provide a digest of a small number of recent interesting papers in the field of translatomics. In this week’s Sunday papers, Hang et al. used ribosome profiling to mechanistically define how plants optimize chloroplast biogenesis and photosynthesis. Liboy-Lugo et al. used ribosome profiling to reveal the effect of isoform-specific translation regulation on stress […]
