Knowledge Hub – Page 10

1. Introduction Ribosome Profiling (Ribo-seq) is a deep-sequencing technique developed by Nicholas Ingolia and Jonathan Weissman that facilitates a genome-wide view of translation in vivo (1).  This...

Translation within the context of viral-host interaction is a crucially important factor in determining the propagation of the invading pathogen. Due to their lack of cellular organelles, it is a requ...

The primary determinants for successful recombinant protein expression are high expression levels of the recombinant genes, translation efficacy and post-translational modifications. Transcriptomic an...

The process of translation is characterized by variation in the local decoding rates of specific mRNA codons. There are known cases where pausing or stalling is used for regulating protein synthesis....

In order to maintain a healthy proteome, the ribosome must not only generate sequences of peptides in sufficient quantities to serve their biological purpose but must also ensure that these polymers c...

Cardiometabolic diseases, significant causal factors in heart attacks and strokes, are the leading cause of death worldwide. With the growing rates of cardiometabolic disorders in the Western world, a...

Micropeptides are peptides that are generally considered to be less than 150 amino acids in length. The identification of these short peptides has been limited by their size, abundance, and the curren...

The dysregulation of translation in the central nervous system (CNS) has been linked to multiple neurodegenerative diseases, making translatomics a particularly important set of tools for learning and...

Multiple translation initiation sites (TISs) may allow for alternative proteoforms to arise from the translation of just a single mRNA. RNA-Seq is limited in that it cannot be utilized to predict alte...

A range of human neurological disorders occur due to nucleotide repeat expansions which can cause disease by protein gain-of-function, protein loss-of-function, or RNA gain-of-function. The FMR1 (Frag...

​Translation is initiated when the small ribosomal subunit, which scans from the 5’ end of the mRNA, recognises a start codon. Identifying sites of translation initiation is desirable for uncoveri...