Understanding Differential Gene Expression (DE) Analysis through Ribosome Profiling and RNA-seq

Introduction In the dynamic landscape of molecular biology, differential gene expression (DE) analysis serves as a cornerstone for understanding the complex regulatory mechanisms underpinning biological processes and disease states. Two revolutionary techniques, ribosome profiling (Ribo-seq) and RNA sequencing (RNA-seq), have emerged as powerful tools for dissecting the nuances of gene expression at an unprecedented resolution. […]

Hitting your target selectively. The potential of EIRNA Bio’s c-MAST Screening platform to rapidly identify off-target effects.

https://eirnabio.com//srv/htdocs/wp-content/uploads/2023/06/Question-40-1.mp4 In the realm of drug discovery, understanding the mechanism of action of therapeutic compounds is crucial, and ribosome profiling (Ribo-seq) has emerged as a powerful tool for this purpose. Despite its strengths, the application of Ribo-seq in a high-throughput manner poses practical limitations. The resource-intensive nature of the technology makes it challenging to triage […]

Translatomics for tRNA, disomes and PBMCs

Recent Publications Harnessing the Power of Translatomics Every week we provide a digest of a small number of recent interesting papers in the field of translatomics. In this week’s Sunday papers, Ando et al. show that tissue-specific tRNA modifications influence codon decoding and translation efficiency. Scazzari et al. demonstrate that stalled disomes are marked by Hel2-dependent ubiquitin chains. […]

Translatomics for chloroplast, stress granules and exonization

Recent Publications Harnessing the Power of Translatomics Every week we provide a digest of a small number of recent interesting papers in the field of translatomics. In this week’s Sunday papers, Hang et al. used ribosome profiling to mechanistically define how plants optimize chloroplast biogenesis and photosynthesis. Liboy-Lugo et al. used ribosome profiling to reveal the effect of isoform-specific translation regulation on stress […]

Translatomics for archaea, E. coli and antimicrobial peptides

Recent Publications Harnessing the Power of Translatomics Every week we provide a digest of a small number of recent interesting papers in the field of translatomics. In this week’s Sunday papers, Tufail et al. used ribosome profiling to discover novel sORFs in Methanosarcina. Dolcemascolo et al. used ribosome profiling to establish Musashi-1 as an orthogonal tool for post-transcriptional gene regulation in E. coli. Fan et al. […]